Detection of Clostridium difficile toxin: comparison of enzyme immunoassay results with results obtained by cytotoxicity assay.

نویسندگان

  • Daniel M Musher
  • Atisha Manhas
  • Pranav Jain
  • Franziska Nuila
  • Amna Waqar
  • Nancy Logan
  • Bernard Marino
  • Edward A Graviss
چکیده

Several kinds of laboratory techniques are available to detect Clostridium difficile toxin in fecal samples. Because questions have been raised about the reliability of immunoassays compared to the accepted standard, cytotoxicity assay, we studied three enzyme immunoassays (EIAs) and one rapid EIA, which demonstrated relatively good sensitivities and specificities compared to cytotoxicity assay.

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منابع مشابه

Evaluation of a new enzyme immunoassay for Clostridium difficile toxin A.

AIM To evaluate a new enzyme immunoassay (EIA) method for detection of Clostridium difficile toxin by comparing it to cytotoxicity assay. To investigate the nature of false negative and false positive EIA results by evaluating clinical and therapeutic parameters. METHODS 737 consecutive diarrhoeal specimens collected from patients clinically suspected of having C difficile colitis were tested...

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Detection of Clostridium difficile toxin : 4 5 Comparison of enzyme immunoassays 6 7 with results obtained by cytotoxicity assay 8 9 10 11 12

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BACKGROUND/AIMS Accurate and rapid laboratory diagnosis of Clostridium difficile infections (CDI) remains a significant challenge. A two-step algorithm for detection of toxigenic C. difficile in stool based on initial screening for glutamate dehydrogenase assay followed by confirmation by toxin A+B detection using an enzyme immunoassay (EIA) or molecular assay has been proposed. We aimed to eva...

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عنوان ژورنال:
  • Journal of clinical microbiology

دوره 45 8  شماره 

صفحات  -

تاریخ انتشار 2007